Investigation of fluorescence’s properties of the ZrO2-2Y2O3-4CeO2+3%Al2O3 nanopowder for further study of the distribution of nanoparticle using imaging method in vivo

Abstract


The increasing use of nanomaterials raises concerns about the long-term effects and chronic exposure to nanoparticles on human health. Among the techniques that are currently the most developed and reliable with regard to detection and identification of synthetic nanoparticles is electron microscopy. It allows determining the number, size, shape, crystalline structure, chemical composition of electron-dense substances in the size range 1-100 nm. However, to examine and identify the distribution and accumulation of engineered nanoparticles in the body of an animal is required not only electron microscopy, as well as a visualization camera, where we can to study the distribution of nanoparticles in the body of the animal in the dynamics at the same time without killing the animal. In the study was used a complex of equipment for the visualization of nanoparticles in the body of the animal. Photon Imager Optima - Bioscape Lab (France) and TECAN SpectraFlour are included in this complex. First one is used for the visualization of static and dynamic processes, when the nanopowders are administered to the laboratory animal. The second one is a multifunctional instrument of measuring of fluorescence and absorption of light. This research will allow better understanding the behavior of the biodistribution of nanoparticles in vivo and allowing the use of the data obtained in the study of the practical use of the toxicity of nanomaterials.

About the authors

I. V Antsiferova

Perm National Research Polytechnic University

E. N Makarova

Perm National Research Polytechnic University

I. V Fefilova

Perm National Research Polytechnic University

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